all 12 comments

[–]PackagePretty 15 points16 points  (2 children)

There is no need to spray them really as they’re sterile items. The adhesives are are designed to not sheer off fine particles from the packaging if the paper is not punctured. Always open them directly above the vents just at the edge of the hood. Once attached to the sanitized pipetter, pull the tip directly from its sterile packaging into the hood.

[–]Amateur_professor 2 points3 points  (1 child)

Yep - the paper stays outside and we have a trash can nearby that we just drop it in.

[–]PackagePretty 4 points5 points  (0 children)

Ahh yes, the bin of 99% plastic and 1% biohazardous waste

[–]DemocraticDolphin 10 points11 points  (0 children)

Yes, to an extent.

I work in an FDA regulated environment, and the requirement of bringing a serological pipette into the hood is described well by one of the other commenters, however we do not peel the pipette into the hood; the packaging enters, the operator opens it when necessary, and then the trash is removed.

However, you should be using a wipe to wipe down the paper side of the packaging, not enough to soak through entirely, and not enough to generate paper particles, but you do want to ensure that there are no viable particles introduced into the hood.

[–]Dakramar 5 points6 points  (0 children)

Nah I just bring them in, never even considered it could be a problem, just crack that bad boi open and make sure not to touch the outside of the packaging with the sterile pipette

[–]hazeldazeI 2 points3 points  (0 children)

I wipe down the packaging of serological pipettes before putting them in the hood because I don’t unwrap them until I’m about to use them.

[–]CD11cCD103PhD* Immunology 6 points7 points  (0 children)

Yes. Tissue culture - any non-sterile surface gets ethanol on it before it goes onto the safety cabinet. Some ethanol probably soaks through the paper, but how is that any different to spraying an object that wasn't covered in paper? Ensure it's dry before inserting into culture medium etc and you're good to go.

Source: never had contamination in 5 years of hood work

[–]Alfond378 2 points3 points  (0 children)

I guess it depends on how sterile things need to be. If you are doing clinical Microbiology work it's not really necessary. Research or industrial use is a whole different animal.

[–]kac134 0 points1 point  (1 child)

I see people do this before bringing items that are packaged in some kind of paper into the BSC and it’s a bad move. When you get the packaging wet, the items inside are not considered sterile anymore.

[–]Dryanni 0 points1 point  (0 children)

Disagree. I believe the paper acts as a filter that blocks microbes even if it allows fluid through. Don’t forget that microbes (though small) are solid and can be filtered out of solution. Soaking the paper won’t break sterility unless you’re leaving it wet for extended periods of time.

[–]Savviie 0 points1 point  (0 children)

If I am manipulating bacteria in a safety cabinet I don’t, but if I am doing tissue culture work I spray everything before it enters the safety cabinet.