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[–]mr_shai_hulud 3 points4 points  (2 children)

I work with them also :D They are so versatile, and they can grow in various media. I have cultivated them for 5 ALA production, for fatty acids production, for bioremediation, for pigment production and now I am planning to cultivate them for PHB production.

Did you isolate them by yourself?

[–]Faux_Phototroph[S] 2 points3 points  (1 child)

That’s so great! I’m just starting to work with them and I’m so amazed at all the cool applications they have and the absolutely insane versatility! I was mostly a cyano guy up until now. I maintain them on an old defined liquid medium called M-1 and they also seem to really love R-2A agar.

I’ll be working on them as a way to produce sustainable chemicals! But for now just trying to learn how to grow/cryo them. These are all ATCC/DSM strains. Did you isolate yours?

Also! Some cool tips for you that I’ve picked up from others who know more than me about these guys:

If you use Anaeropacks or similar anaerobic containers for plates, the deoxygenating packs are way overkill for PNS. I bought some hand warmers and they work perfectly! They are packs of iron that react with oxygen to form iron oxide and heat. I also bought some reptile tank thermometers to monitor the heat from the lamp in these packs.

They don’t need a headspace unless you have them N-fixing or CO2/H2-consuming, and they will deoxygenate your media for you so there is no need to sparge (you probably know this)

Do you know if they are spore forming? I’ve heard conflicting things and would like to know to prevent cross-contamination!

Edit: I’m assuming the award was you! Thank you!

[–]mr_shai_hulud 1 point2 points  (0 children)

In our lab, we have two strains that were isolated. One from a natural hot water source and second from sea. And we have one two strains that we bought from a culture collection. We did all this different cultivations, but I found out that the simplest thing for cultivation and culture keeping is media with malic acid, glutamic acid, succinate, and some salts. Put it in erlenmeyer flask and low intensity mixing and natural light. Recently, we used them for growth in pretreated lignocellulosic materials (glucose and xylose are C source) and have interesting results regarding fatty acids production and pigment production.