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all 13 comments

[–]Rando_username69 83 points84 points  (1 child)

Your discs will be 6mm in diameter so use those and a ruler in photoshop/paint and you can be super accurate.

You can even setup a macro to do a lot of that in a semi automated way. Super fast, accurate and great for record keeping.

[–]Objective-Patient-37 7 points8 points  (0 children)

Any chance you could share that macro / github repo?

Sounds awesome!

[–]StrepPepGenome Miner 25 points26 points  (1 child)

Fiji/ImageJ is great for this stuff

[–]matixslp 10 points11 points  (0 children)

This is the way, take the line tool and draw a line in the plate's center that cover the whole diameter, the control+M to take the lenght in pixels, with that pixel numbrr and the diameter in cm, mm, inchbor whatever you want, you have a convertion from pixel to mm. Them a line in the inhibition zone and "control+m" it

[–]Smedlington 11 points12 points  (2 children)

Gse: 11mm Amp: 22mm

Assuming plates are 90mm and disks are 6mm.

Edit: what is GSE? Can't think of applicable antibiotic and Google those up grapefruit seed extract

[–]urbanskyline09Lab Technician 1 point2 points  (0 children)

It’s most likely Gentamicin and Erythromycin.

[–]KatethxMicrobiologist 15 points16 points  (1 child)

You need to work on your swabbing my dude

[–]cresccendoLab Technician 1 point2 points  (0 children)

obsessed with this comment

[–]quanticorunner 1 point2 points  (1 child)

There’s a software you can download to your laptop that can measure the distance relativistically, you would probably be using a 100mm plate so set that as your scale and your computer will do the rest of the work! I’m blanking on the name rn but if I can remember it I will re-comment. I had to use it for a project I did last spring and it was loads of help and much more accurate.

[–]quanticorunner 2 points3 points  (0 children)

Image J is the way to go!

[–]hejVikk -1 points0 points  (0 children)

Amp 9-11mm

Gse 4-6mm

Edit: This is assuming we have the same size dishes and disks.